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Abstract Background: Intra operative cytology is a rapid, cheap, nonaggressive and valuable method for diagnosis of breast lesions. Objective: To assess the diagnostic accuracy of intra operative cytology of breast lesions. Methods: This prospective study was carried out on 70 specimens referred to pathology lab in Shahid Rajaie hospital. Findings: Out of total specimens, 37 were benign, 4 diagnosed as suspected malignancy associated with hypercellularity and moderate cell atypia, 29 malignant, 3 misdiagnosed due to hypercellularity and absence of cellular atypia. All 4 inflammatory lesions were diagnosed correctly. Diagnostic accuracy of benign, malignant and inflammatory lesions was 90%, 90% and 100%, respectively. Conclusion: Intra operative cytology is a rapid, cheap and valuable method in evaluating the entire margin of a tumoral mass and with good results in maintaining the physical appearance (cosmetic). It also helps for a better management of breast lesions

Keywords: Keywords: Intra Operative Cytology (IOC), Breast

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