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Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Iran , zeinoddini@modares.ac.ir
Abstract:   (223 Views)
The Coronavirus Disease 2019 that named COVID-19 is a world problem and according to declaration of the World Health Organization (WHO) is a new and extreme outbreak that spreading in all of countries. Firstly, many patients reported exposure in Wuhan city (China) at a large animal stores and seafood. For this, the first idea is suggesting animal-to-human transmission of this infection pathogen. In follow, since then many patients have decelerated no exposure to animal shops, indicating that human-to-human transmission is occurring. The new coronavirus (2019-nCoV) is a positive RNA virus with 29.8 kb genome and S, E, M, N and Orf1 gene fragments. The most popular a method for 2019-nCoV detection is genome-based detection such as PCR based that they require expensive experimental equipments, controlled working environment and high-trained technician, which are often lacking in massive viral outbreaks. Therefore, other rapid and simple genome-based assay was evaluated for this pathogen. In this review study, RT-LAMP technique as main isothermal amplification assays with less time-consuming and without need to expensive equipment than conventional PCR-based methods in order to 2019-nCoV identification, was discussed and advantage or disadvantage of these techniques was compared. The data was indicated, this molecular and isothermal method could be used as one step process successfully for portable screening and rapid identification of 2019-nCoV.
     
Type of Study: Review article | Subject: Virology

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