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Shabani M, Daryanoosh F, Salesi M, Kooshki Jahromi M, Fallahi A A. Effect of continuous training on the level of PPAR-γ and PRDM16 proteins in adipose tissue in overweight diabetes rats. Journal of Inflammatory Diseases. 2018; 22 (3) :4-12
URL: http://journal.qums.ac.ir/article-1-2627-en.html
1- Department of Physical Education, School of Education and Psychology, Shiraz University, Shiraz, Iran
2- Department of Physical Education, School of Education and Psychology, Shiraz University, Shiraz, Iran , daryanoosh@shirazu.ac.ir
Abstract:   (5441 Views)
Background: PPAR-γ and PRDM16 proteins have key role in the metabolism of adipose tissue and the conversion of white tissue to brown adipose tissue. But, the role of exercise on these two important proteins has not been studied in subcutaneous adipose tissue.
Objective: The aim of this study was to investigate the effect of
continuous training 
on the level of PPAR-γ and PRDM16 proteins in the adipose tissue in overweight male Sprague-Dawley with diabetes.
Methods: In this study, 16 two-month old Sprague-Dawley rats with an average weight of 270±20 g were selected and randomly divided into two groups: control (n=8) and continuous training (n=8). The training group exercised according to the training program 4 days a week for 8 weeks while the control group did not have a training program. Independent t-test was used to analyze the data.
Findings: There was a significant increase in the expression of PPAR-γ (P=0.004) and PRDM16 (P=0.0001) proteins in the training group compared to control group.
Conclusion: Considering the increase of PPAR-γ and PRDM16 proteins in adipose tissue after continuous exercise and the important role of these two proteins in the fat metabolism, aerobic exercise can be an important mechanism for reducing this tissue in obese individuals and converting white tissue to brown.
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Type of Study: Research | Subject: physical education

1. Bornfeldt KE, Tabas I. Insulin resistance, hyperglycemia, and atherosclerosis. Cell Metab 2011; 14(5): 575-85. doi: org/10.1016/ j.cmet.2011.07.015. [DOI] [PubMed]
2. Warner A, Mittag J. Breaking BAT: can browning create a better white? J Endocrinol 2016; 228(1): R19-29. [DOI] [PubMed]
3. Zafrir B. Brown adipose tissue: research milestones of a potential player in human energy balance and obesity. Horm Metab Res 2013; 45(11): 774-85. doi: org/10.1055/s-0033-1348264. [PubMed]
4. Harms M, Seale P. Brown and beige fat: development, function and therapeutic potential. Nat Med 2013; 19(10): 1252-63. [DOI] [PubMed]
5. Seale P, Conroe HM, Estall J, Kajimura S, Frontini A, Ishibashi J, et al. Prdm16 determines the thermogenic program of subcutaneous white adipose tissue in mice. J Clinl Invest 2011; 121(1): 96-105. doi: org/10. 1172/JCI44271. [PubMed]
6. Kajimura S, Seale P, Kubota K, Lunsford E, Frangioni JV, Gygi SP, et al. Initiation of myoblast to brown fat switch by a PRDM16–C/EBP-β transcriptional complex. Nature 2009; 460(7259): 1154-8. doi: 10.1038/ nature08262. [PubMed]
7. Cohen P, Levy JD, Zhang Y, Frontini A, Kolodin DP, Svensson KJ, et al. Ablation of PRDM16 and beige adipose causes metabolic dysfunction and a subcutaneous to visceral fat switch. Cell 2014; 156(1-2): 304-16. [DOI] [PubMed]
8. Harms MJ, Lim HW, Ho Y, Shapira SN, Ishibashi J, Rajakumari S, et al. PRDM16 binds MED1 and controls chromatin architecture to determine a brown fat transcriptional program. Genes Dev 2015; 29(3): 298-307. doi: 10.1101/gad.252734. 114. [PubMed]
9. Lo KA, Sun L. Turning WAT into BAT: a review on regulators controlling the browning of white adipocytes. Biosci Rep 2013; 33(5): e00065. [DOI] [PubMed]
10. Koppen A, Kalkhoven E. Brown vs white adipocytes: the PPARγ coregulator story. FEBS Lett 2010; 584(15): 3250-9. [DOI] [PubMed]
11. Kajimura S, Spiegelman BM, Seale P. Brown and beige fat: physiological roles beyond heat generation. Cell Metab 2015; 22(4): 546-59. [DOI] [PubMed]
12. Porcari J, Bryant C, Comana F. Exercise physiology. FA Davis; 2015. 36-62.
13. Ringholm S, Grunnet Knudsen J, Leick L, Lundgaard A, Munk Nielsen M, Pilegaard H. PGC-1α is required for exercise-and exercise training-induced UCP1 up-regulation in mouse white adipose tissue. PloS One 2013; 8(5): e64123. [DOI] [PubMed]
14. Liu WX, Wang T, Zhou F, Wang Y, Xing JW, Zhang S, et al. Voluntary exercise prevents colonic inflammation in high-fat diet-induced obese mice by up-regulating PPAR-γ activity. Biochem Biophys Res Commun 2015; 459(3): 475-80. [DOI] [PubMed]
15. Pierre W, Gildas AJ, Ulrich MC, Modeste WN, Benoît NT, Albert K. Hypoglycemic and hypolipidemic effects of Bersama engleriana leaves in nicotinamide/ streptozotocin-induced type 2 diabetic rats. BMC Complement Altern Med 2012; 12: 264. [DOI] [PubMed]
16. Shirwaikar A, Rajendran K, Barik R. Effect of aqueous bark extract of Garuga pinnata Roxb. in streptozotocin-nicotinamide induced type-II diabetes mellitus. J Ethnopharmacol 2006; 107(2): 285-90. [DOI] [PubMed]
17. Burniston JG. Adaptation of the rat cardiac proteome in response to intensity‐controlled endurance exercise. Proteomics 2009; 9(1): 106-15. [DOI] [PubMed]
18. Haram PM, Kemi OJ, Lee SJ, Bendheim MØ, Al-Share QY, Waldum HL, et al. Aerobic interval training vs. continuous moderate exercise in the metabolic syndrome of rats artificially selected for low aerobic capacity. Cardiovasc Res 2009; 81(4): 723-32. doi: org/10.1093/cvr/cvn332. [PubMed]
19. Tjønna AE, Lee SJ, Rognmo Ø, Stølen TO, Bye A, Haram PM, et al. Aerobic interval training versus continuous moderate exercise as a treatment for the metabolic syndrome: a pilot study. Circulation 2008; 118(4): 346-54. [DOI] [PubMed]
20. Fatone C, Guescini M, Balducci S, Battistoni S, Settequattrini A, Pippi R, et al. Two weekly sessions of combined aerobic and resistance exercise are sufficient to provide beneficial effects in subjects with Type 2 diabetes mellitus and metabolic syndrome. J Endocrinol Invest 2010; 33(7): 489-95. doi: org/10.1007/BF03346630. [PubMed]
21. Petridou A, Tsalouhidou S, Tsalis G, Schulz T, Michna H, Mougios V. Long-term exercise increases the DNA binding activity of peroxisome proliferator–activated receptor γ in rat adipose tissue. Metabolism 2007; 56(8): 1029-36. [DOI] [PubMed]
22. Jeremic N, Chaturvedi P, Tyagi SC. Browning of white fat: novel insight into factors, mechanisms, and therapeutics. J Cell Physiol 2017; 232(1): 61-8. [DOI] [PubMed]

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