Volume 17, Issue 4 (Oct- Nov 2013)                   2013, 17(4): 11-16 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Naserpour Farivar T, Naderi M, Johari P. Frequency of NRAMP-1 Gene Polymorphism at INT4, D543 and 3'UTR loci in Tuberculosis patients and control group in Zahedan . Journal of Inflammatory Diseases. 2013; 17 (4) :11-16
URL: http://journal.qums.ac.ir/article-1-1474-en.html
1- , Email:tnaserpour@qums.ac.ir
2- Zahedan University of Medical Sciences
3- Qazvin University of Medical Sciences,Cellular and Molecular Research Center
Abstract:   (7662 Views)

  Background: The natural resistance-associated macrophage protein 1 ( NRAMP-1) gene encodes a protein which finally kills Mycobacterium tuberculosis. Susceptibility to tuberculosis may be influenced by mutations in this gene.

  Objective: The aim of this study was to compare the frequency of NRAMP-1 gene polymorphisms among tuberculosis patients and control group  in Zahedan.

  M et h o d s : This molecular epidemiologic study was conducted on 82 tuberculosis patients and 150 healthy controls referred to Booali Hospital in Zahedan, during 2012. After Polymerase chain reaction (PCR), PCR products were treated by AvaII, FokI and ApaI restriction enzymes and were evaluated by restriction fragment length polymorphism (RFLP) to determine the NRAMP-1 gene polymorphisms at INT4, D543 and 3' UTR loci. Data were analyzed by Fisher Exact test and descriptive statistics.

  Findings: The heterozygote patterns at INT4 (G/C), D543 (G/A) and 3'UTR (+/del) loci were more prevalent in the controls than in patients while the homozygous patterns at these loci were more prevalent in patients.

  C o nclusio n : This study showed that individuals with homozygous type mutation have an increased risk of developing tuberculosis compared to heterozygote ones.



Full-Text [PDF 239 kb]   (1730 Downloads)    
Type of Study: Research | Subject: Microbiology

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Journal of Inflammatory Diseases

Designed & Developed by : Yektaweb