Volume 5, Issue 1 (Spring 2001)                   J Qazvin Univ Med Sci 2001, 5(1): 55-60 | Back to browse issues page

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Afshari A, Holme E. Introducing a new method to detect bound C3d on erythrocytes during complement activation. J Qazvin Univ Med Sci. 2001; 5 (1) :55-60
URL: http://journal.qums.ac.ir/article-1-511-en.html
Abstract:   (6712 Views)
Background: Measurement of complement activation products has recently been the popular means of assessing the complement activation. Objective: To introduce a new method to detect bound C3d on erythrocytes during complement activation and to compare it with free C3d levels. Methods: An indirect ELISA used in routine measurement of free C3d levels was developed together with the modified ELISA. To capture bound C3d on erythrocytes, ELISA plate were coated with anti-C3d and after incubation, blood samples were added for two hours. Bound erythrocytes were lysed with 100µl water perwell and the OD were read at 405nm. Findings: The levels of free C3d and bound C3d on the erythrocytes during complement activation was significantly increased compared to the control (800% and 500% respectively). Conclusion: Both free C3d and bound C3d as final dogradation product of C3d reflect the ongoing complement activation.
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Type of Study: Research | Subject: Immunology

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