Volume 8, Issue 3 (Autumn 2004)                   J Qazvin Univ Med Sci 2004, 8(3): 3-7 | Back to browse issues page

XML Persian Abstract Print

1- , Email: ToKmeh@yahoo. com
Abstract:   (6934 Views)
¯Abstract Background: Zinc has important effects on structural and functional activities of many proteins and enzymes, specially regulation of immune system. Objective: This study was carried out to examine the in vitro effects of different concentration of zinc on viability and morphology of Raji cell line. Methods: In this study the cell line was exposed to different concentration of zinc(10nM to 500µM)followed by incubation (37 c, 5%Co2) at various time points(12 to 72 hrs). The cells were then evaluated with trypan blue exclusion dye , and Wright-Gimsa staining. Findings: The results showed almost different responses to different amount of zinc by the Raji cells. less than 100µM at different incubation time points had no effects on cell line when compared to the controls. Higher concentrations of zinc (>100µM) viability diminished to 70% at 12 hrs and less than 50% at 24 hrs of incubation times . Conclusion: We conclude that Zn has dose-dependent cytotoxic effect on Raji cells and probably application for immune-modulation.
Full-Text [PDF 145 kb]   (1461 Downloads)    
Type of Study: Research | Subject: Immunology