Volume 19, Issue 1 ( Apr-May 2015)                   J Qazvin Univ Med Sci 2015, 19(1): 24-31 | Back to browse issues page

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Baharvand R. Prf A gene in Listeria monocytogenes isolated from food. J Qazvin Univ Med Sci. 2015; 19 (1) :24-31
URL: http://journal.qums.ac.ir/article-1-1704-en.html
, Email:baharkia44@yahoo.com
Abstract:   (3241 Views)

  Background: Listeria is a gram-positive bacterium and a facultative food- borne intracellular pathogen. Prf A gene regulates the expression of other genes and is important in virulence.

  Objective: The aim of this study was to identify Prf A gene in Listeria monocytogenes isolated from food samples.

  Methods: This cross - sectional study was conducted in 212 different food samples collected from different cities in 2012. Listeria monocytogenes was isolated using the cold enrichment method. The Prf A gene was identified using polymerase chain reaction (PCR). Data were analyzed using Chi- square test.

  Findings: Of 212 samples, 41 (%19.33) were positive for Listeria spp. Of 41, 22 (%53.6) were Listeria monocytogenes, 15 (%36.5) were L. innocua, 3 (%7.3) were L. welshimri and 1 (%2.4) was L. seeligeri . The Prf A gene was found in 28.5% of L. monocytogenes isolated from vegetable samples and in 100% L. monocytogenes isolated from other samples.

  Conclusion: Confirmation of the presence of L. monocytogenes in food and the Prf A gene may be helpful to prevent diseases caused by Listeria .


  Citation: Baharvand R. Prf A gene in Listeria monocytogenes isolated from food. J Qazvin Univ Med Sci. 2015 19 (1): 24-31.







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Type of Study: Research | Subject: Microbiology

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