Volume 8, Issue 4 (Winter 2005)                   J Qazvin Univ Med Sci 2005, 8(4): 21-26 | Back to browse issues page

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Shams Asanjan K. A survey on platelet surface glycoprotiens in patients with Bernard-Souliar syndrome by flowcytometry . J Qazvin Univ Med Sci. 2005; 8 (4) :21-26
URL: http://journal.qums.ac.ir/article-1-164-en.html
, Email: Sh.Asengan@gmail.com
Abstract:   (8520 Views)
Abstract Background: The gold standard diagnosis of the Bernard-Souliar syndrome (BSS), a rare disease, is to prove the absence of Ib/IX surface complex on platelets with the use of aggregometric methods. Flowcytometry is an ideal method in analysis of surface markers on cells. Objective: The use of flowcytometric analysis in diagnosis of Bernard-Souliar syndrome. Methods: 15 suspected BSS, 20 healthy persons as control group and 3 ITP patints were selected to be analysed for the presence of GPIbα and GPIIIa on the surface of platelets with the application of FITC conjugated monoclonal antibodies using flowcytometry. Findings: All healthy persons in control group and 3 ITP patients showed normal expression of both glycoprotiens on platelets using flowcytometry. All 15 suspected BSS patients showed lack of GPIbα but a normal expression of GPIIIa on platelets. Conclusion: The application of flowcytometry for diagnosis of BSS is a quick, accurate, and precise method, which together with aggregometric method can be used for diagnosis of BSS.
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Type of Study: Research | Subject: Clinical Laboratory

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