Volume 4, Issue 4 (Winter 2001)                   J Qazvin Univ Med Sci 2001, 4(4): 14-22 | Back to browse issues page

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Mirabzadeh Ardakani E. The rapid method of preparing mouse karyotype and mouse chromosomes identified by Gimsa staining and Trypsin-Gimsa banding. J Qazvin Univ Med Sci. 2001; 4 (4) :14-22
URL: http://journal.qums.ac.ir/article-1-631-en.html
Abstract:   (13935 Views)
Abstract Background: Preparation and staining of mouse chromosomes by Gimsa and T-G banding is the cornerstone of many researches on diagnosis, prevention and treatment of many diseases, production of new drugs and quality control of biological derivatives. Objective: To provide a sample karyotype of mouse. Methods: Mouse chromosomes were prepared from bone marrow of male outbred mice (NMRI). After culturing and preparing the slides, they were studied by staining with Gimsa and Trypsin-Gimsa (T-G) banding techniques. Karyotypes were prepared according to the standard karyotype of the mouse. Findings: Metaphase with spread from diploid cells contained 40 chromosomes and all of them were characterized by their banding patterns. Conclusion: T-G technique provides greather detail and demonstrates more bands, in contrast with other methods.
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Type of Study: Research | Subject: ENT

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