Volume 21, Issue 6 (Feb - Mar 2018)                   J Qazvin Univ Med Sci 2018, 21(6): 37-30 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hajifathaliya Z, Najafipour R, Modarressi M, Savad S, Rashvand Z. Association between the HLA-G*0105N polymorphism and recurrent abortion in women. J Qazvin Univ Med Sci. 2018; 21 (6) :37-30
URL: http://journal.qums.ac.ir/article-1-2337-en.html
Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (838 Views)

Background: In human pregnancies HLA-G (Human leukocyte antigens G) is supposed that play a main role in implantation. It is expressed on trophoblast cells that invades during pregnancy to the uterus decidua and is introduced to induce maternal tolerance immunologically against the fetus.
Objective: The aim of this study was to find association between the HLA-G*0105N polymorphism and recurrent abortion in women.
Methods: This case-control study was conducted on two groups of 108 women with history recurrent abortion (RSA) as a case group or without history RSA as a control group in Cellular and Molecular Research Center, Qazvin University of Medical Sciences in 2015. The frequency of null allele of HLA-G*0105N was studied in two groups by PCR -RFLP method. Allele and genotype frequencies of two group were compared using Hardy-Weinberg equilibrium and Chi-square test.
Findings: In case group, 54 (50%) and 17 (15.7%) were heterozygous and homozygous respectively for HLA-G*0105N polymorphism. In control group, only 9 (8%) was homozygous for HLA-G*0105N polymorphism. But there was no significant difference between case and control on HLA-G*0105N allele with RSA (P=0.1004).
Conclusion: As the results, there was no significant association between HLA-G*0105N poly morphism and RSA. Thus, it seems that this HLA-G*0105N polymorphism may effective in relation with other genes or polymorphisms in RSA.

Full-Text [PDF 299 kb]   (153 Downloads)    
Type of Study: Research | Subject: Genetics

References
1. Berger DS, Hogge WA, Barmada MM, Ferrell RE. Comprehensive analysis of HLA-G: implications for recurrents pontaneous abortion. Reprod Sci 2010; 17(4): 331-8. [DOI] [PubMed]
2. Akhter A, Faridi RM, Das V, Pandey A, Naik S, Agrawal S. In vitro up-regulation of HLA-G using dexamethasone and hydrocortisone in first-trimester trophoblast cells of women experiencing recurrent miscarriage. Tissue Antigens 2012; 80(2): 126-35. [DOI] [PubMed]
3. Mosaferi E, Majidi J, Mohammadian M, Babaloo Z, Monfaredan A, Baradaran B. HLA-G expression pattern: reliable assessment for pregnancy outcome prediction. Adv Pharm Bull 2013; 3(2): 443-6. doi: 10.5681/apb.2013.072. [PubMed]
4. Donadi EA, Castelli EC, Arnaiz-Villena A, Roger M, Rey D, Moreau P. Implications of the polymorphism of HLA-G on its function, regulation, evolution and disease association. Cell Mol Life Sci 2011; 68(3): 369-95. [DOI] [PubMed]
5. Rizzo R, Vercammen M, van de Velde H, Horn PA, Rebmann V. The importance of HLA-G expression in embryos, trophoblast cells, and embryonic stem cells. Cell Mol Life Sci 2011; 68(3): 341-52. doi: 10.1007/ s00018-010-0578-1. [PubMed]
6. González A, Rebmann V, LeMaoult J, Horn PA, Carosella ED, Alegre E. The immunosuppressive molecule HLA-G and its clinical implications. Crit Rev Clin Lab Sci 2012; 49(3): 63-84. doi: 10.3109/10408363. 2012.677947. [PubMed]
7. Rizzo R, Bortolotti D, Baricordi OR, Fainardi E. New insights into HLA-G and inflammatory diseases. Inflamm Allergy Drug Targets 2012; 11(6): 448-63. [DOI] [PubMed]
8. Le Page ME, Goodridge JP, John E, Christiansen FT, Witt CS. Response to comment on "Killer Ig-like receptor 2DL4 does not mediate NK cell IFN-gamma responses to soluble HLA-G preparations. J Immunol 2014; 192(9): 4003-4. [DOI] [PubMed]
9. Gregori S, Tomasoni D, Pacciani V, Scirpoli M, Battaglia M, Magnani CF, et al. Differentiation of type1 T regulatory cells (Tr1) by tolerogenic DC-10 requires the IL-10-dependent ILT4/HLA-G pathway. Blood 2010; 116(6): 935-44. [DOI] [PubMed]
10. Catamo E, Addobbati C, Segat L, Sotero Fragoso T, Tavares Dantas A, de Ataide Mariz H, et al. Comprehensive analysis of polymorphisms in the HLA-G 5′upstream regulatory and 3′untranslated regions in Brazilian patients with systemic lupus erythematosus. Tissue Antigens 2015; 85(6): 458-65. [DOI] [PubMed]
11. Abbas A, Tripathi P, Naik S, Agrwal S. Analysis of human leukocyte antigen(HLA)–G polymorphism in normal women and in women with recurrent spontaneous abortions. Eur J Immunogenet 2004; 31(6): 275-8. [DOI] [PubMed]
12. Roberta R, Daria B, Silvia B, Enrico F. HLA-G molecules in autoimmune diseases and infections. Front Immunol 2014; 5: 592. [DOI] [PubMed]
13. Le Discorde M, Le Danff C, Moreau P, Rouas-Freiss N, Carosella ED. HLA-G*0105N null allele encodes functional HLA-G isoforms. Biol Reprod 2005; 73(2): 280-8. [DOI] [PubMed]
14. Simon A, Laufer N. Repeated implantation failure: clinical approach. Fertil Steril 2012; 97(5): 1039-43. [DOI] [PubMed]
15. Dahl M, Hviid TV. Human leucocyte antigen class Ib molecules inpregnancy success and early pregnancy loss. Hum Reprod Update 2012; 18(1): 92‑109. [DOI] [PubMed]
16. Verloes A, Van de Velde H, LeMaoult J, Mateizel I, Cauffman G, Horn PA, et al. HLA-G expression in human embryonic stem cells and preimplantation embryos. J Immunol 2011; 186(4): 2663-71. [DOI] [PubMed]
17. Zidi I, Rizzo R, Bouaziz A, Laaribi AB, Zidi N, Di Luca D, et al. sHLA-G1 and HLA-G5 levels are decreased in Tunisian women with multiple abortion. Hum Immunol 2016; 77(4): 342-5. [DOI] [PubMed]
18. Nardi Fda S, Slowik R, Wowk PF, da Silva JS, Gelmini GF, Michelon TF, et al. Analysis of HLA-G polymorphisms in couples with implantation failure. Am J Reprod Immunol 2012; 68(6): 507-14. [DOI] [PubMed]
19. Jassem RM, Shani WS, Loisel DA, Sharief M, Billstrand C, Ober C. HLA-G polymorphisms and soluble HLA-G protein levels in women with recurrent pregnancy loss from Basrah province in Iraq. Hum Immunol 2012; 73(8): 811-7. doi: 10.1016/j. humimm.2012.05.009. [PubMed]
20. Morandi F, Pistoia V. Interactions between HLA-G and HLA-E in physiological and pathological conditions. Front Immunol 2014; 5: 394-9. doi: 10.3389/fimmu.2014. 00394. [PubMed]
21. Ober C, Aldrich CL, Chervoneva I, Billstrand C, Rahimov F, Gray HL, et al. Variation in the HLA-G promoter region influences miscarriage rates. Am J Hum Genet 2003; 72(6): 1425-35. [DOI] [PubMed]
22. Enghelabifar M, Allafan S, Khayatzadeh J, Shahrokh Abadi Kh, Hasanzadeh Nazarabadi M, Moradi F, et al. Association of the maternal 14-bp insertion/deletion polymorphism in the histocompatibility leukocyte antigen G gene with recurrent implantation failure. Iran J Reprod Med 2014; 12(9): 641-6. [PubMed]
23. Jeong KH, Kim SK, Kang BK, Chung JH, Shin MK, Lee MH. Association between an HLA-G 14 bp insertion/deletion polymorphism and non-segmental vitiligo in the Korean population. Arch Dermatol Res 2014; 306(6): 577-82. [DOI] [PubMed]
24. Barrientos G, Toro A, Moschanskyb P, Cohen M, Garcia MG, Rose B, et al. Leptin promotes HLA-G expression on placental trophoblasts via the MEK/Erk and PI3K signaling pathways. Placenta 2015; 36(4): 419-26. [DOI] [PubMed]
25. 25. Rahimi R, Zavaran Hosseini A, Yari F. Null allele frequencies at HLA-G locus in Iranian healthy subjects. Iran J Immunol 2008; 5(4): 207-11. doi: IJIv5i4A3. [PubMed]

Add your comments about this article : Your username or Email:
CAPTCHA code

© 2018 All Rights Reserved | The Journal of Qazvin University of Medical Sciences

Designed & Developed by : Yektaweb